Assay Nameβ
dPCR Mutation Assay KRAS 534 Human
GeneGlobe Cat No (Assay ID)β
DMH0000527
Speciesβ
Human (Homo sapiens)
Gene Symbolβ
KRAS
Gene aliasesβ
KRAS1;K-Ras4B;KRAS2
Ensembl Gene IDβ
ENSG00000133703
Entrez Gene IDβ
3845
Genomic Mutation ID (COSV by COSMIC)β
COSV55522580
Legacy Mutation ID (COSM by COSMIC)β
COSM534
Amino Acid Changeβ
p.G13V
Nucleotide Changeβ
c.38G>T
Wildtype Alleleβ
C
Mutant Alleleβ
A
Mutation Strandβ
-
Mutant descriptionβ
Substitution - Missense
Positive Control Sequenceβ
AATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAATAATG
Amplicon lengthβ
91
Recommended Restriction Enzymeβ
CviQI,EcoRI,XbaI,PvuII
Wet-lab validatedβ
dPCR wet-lab validated
Probe Fluorophoreβ
FAM/HEX or ATTO550/ROX
Quencherβ
Iowa Black
Primer Purificationβ
Desalted
Probe Purificationβ
HPLC