Assay Nameβ
dPCR Mutation Assay KRAS 1159597 Human
GeneGlobe Cat No (Assay ID)β
DMH0000290
Speciesβ
Human (Homo sapiens)
Gene Symbolβ
KRAS
Gene aliasesβ
KRAS1;K-Ras4B;KRAS2
Ensembl Gene IDβ
ENSG00000133703
Entrez Gene IDβ
3845
Genomic Mutation ID (COSV by COSMIC)β
COSV55502066
Legacy Mutation ID (COSM by COSMIC)β
COSM1159597
Amino Acid Changeβ
p.Q61K
Nucleotide Changeβ
c.181C>A
Wildtype Alleleβ
G
Mutant Alleleβ
T
Mutation Strandβ
-
Mutant descriptionβ
Substitution - Missense
Positive Control Sequenceβ
CCCTCCCCAGTCCTCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCAAGAGACAGGTTTCTCCATCAATTACT
Amplicon lengthβ
79
Recommended Restriction Enzymeβ
AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validatedβ
dPCR wet-lab validated
Probe Fluorophoreβ
FAM/HEX or ATTO550/ROX
Quencherβ
Iowa Black
Primer Purificationβ
Desalted
Probe Purificationβ
HPLC