Assay Nameβ
dPCR Mutation Assay IDH1 28750 Human
GeneGlobe Cat No (Assay ID)β
DMH0000015
Speciesβ
Human (Homo sapiens)
Gene Symbolβ
IDH1
Ensembl Gene IDβ
ENSG00000138413
Entrez Gene IDβ
3417
Genomic Mutation ID (COSV by COSMIC)β
COSV61615420
Legacy Mutation ID (COSM by COSMIC)β
COSM28750
Amino Acid Changeβ
p.R132L
Nucleotide Changeβ
c.395G>T
Wildtype Alleleβ
C
Mutant Alleleβ
A
Mutation Strandβ
-
Mutant descriptionβ
Substitution - Missense
Positive Control Sequenceβ
TCACATTATTGCCAACATGACTTACTTGATCCCCATAAGCATGACGACCTATGATGATAGGTTTTACCCATCCACTCACAAGCCGG
Amplicon lengthβ
66
Recommended Restriction Enzymeβ
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validatedβ
dPCR wet-lab validated
Probe Fluorophoreβ
FAM/HEX or ATTO550/ROX
Quencherβ
Iowa Black
Primer Purificationβ
Desalted
Probe Purificationβ
HPLC