Assay Name
dPCR Mutation Assay KRAS 521 Human
GeneGlobe Cat No (Assay ID)
DMH0000286
Species
Human (Homo sapiens)
Gene Symbol
KRAS
Gene aliases
KRAS1;K-Ras4B;KRAS2
Ensembl Gene ID
ENSG00000133703
Entrez Gene ID
3845
Genomic Mutation ID (COSV by COSMIC)
COSV55497369
Legacy Mutation ID (COSM by COSMIC)
COSM521
Amino Acid Change
p.G12D
Nucleotide Change
c.35G>A
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
ATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAATA
Amplicon length
87
Recommended Restriction Enzyme
CviQI,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC